Heterogeneous Fenton’s-like Catalyst Potentiation of Hydrogen Peroxide Disinfection: an Investigation into Mechanisms of Action

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dc.cclicenceN/Aen
dc.contributor.authorSewell, S
dc.contributor.authorOwen, Lucy
dc.contributor.authorHuddersman, Katherine
dc.contributor.authorLaird, Katie
dc.contributor.authorWalsh, Susannah
dc.date.acceptance2022-11
dc.date.accessioned2023-03-06T09:39:56Z
dc.date.available2023-03-06T09:39:56Z
dc.date.issued2023-02-16
dc.descriptionopen access articleen
dc.description.abstractAims This study aimed to establish the mechanisms of action (MOA) of a novel surface-functionalized polyacrylonitrile (PAN) catalyst, which was previously shown to have potent antimicrobial activity in conjunction with hydrogen peroxide (H2O2). Methods and results Bactericidal activity was determined using a disinfectant suspension test. The MOA was investigated by measuring the loss of 260 nm absorbing material, membrane potential, permeability assays, analysis of intra- and extracellular ATP and pH, and tolerance to sodium chloride and bile salts. The catalyst lowered sub-lethal concentrations of H2O2 from 0.2 to 0.09%. H2O2 ± 3 g PAN catalyst significantly (P ≤ 0.05) reduced sodium chloride and bile salt tolerance, suggesting the occurance of sublethal cell membrane damage. The catalyst significantly increased (P ≤ 0.05) N-Phenyl-l-Napthylamine uptake (1.51-fold) and leakage of nucleic acids, demonstrating increased membrane permeability. A significant (P ≤ 0.05) loss of membrane potential (0.015 a.u.), coupled with pertubation of intracellular pH homeostasis and depletion of intracellular ATP, suggests potentiation of H2O2-mediated cell membrane damage. Conclusions This is the first study to investigate the catalyst’s antimicrobial mechanism of action, with the cytoplasmic membrane being a target for cellular injury.en
dc.funderNo external funderen
dc.identifier.citationSewell, S., Owen, L., Laird, K., Huddersman, K. and Walsh, S. (2022) Heterogeneous Fenton’s-like Catalyst Potentiation of Hydrogen Peroxide Disinfection: an Investigation into Mechanisms of Action. Journal of Applied Microbiology, 134 (3), lxad017en
dc.identifier.doihttps://doi.org/10.1093/jambio/lxad017
dc.identifier.issn1365-2672
dc.identifier.urihttps://hdl.handle.net/2086/22562
dc.language.isoenen
dc.peerreviewedYesen
dc.publisherOxford University Pressen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.titleHeterogeneous Fenton’s-like Catalyst Potentiation of Hydrogen Peroxide Disinfection: an Investigation into Mechanisms of Actionen
dc.typeArticleen

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