Polythiophene nanofilms for sensitive fluorescence detection of viruses in drinking water

dc.cclicenceCC-BY-NCen
dc.contributor.authorWankar, Shashwatien
dc.contributor.authorTurner, Nicholas W.en
dc.contributor.authorKrupadam, Reddithota J.en
dc.date.acceptance2016-03-10en
dc.date.accessioned2018-11-01T14:29:17Z
dc.date.available2018-11-01T14:29:17Z
dc.date.issued2016-03-11
dc.descriptionThe author's final peer reviewed version can be found by following the URI link.. The Publisher's final version can be found by following the DOI link.en
dc.description.abstractMolecular imprints of the tobacco necrosis virus (TNV) have been formed within polythiophene nanofilms with an approximate thickness of 200 nm. These films have been electrochemically deposited onto conducting Au surfaces. Upon rebinding, the TNV-polythiophene complex changes the fluorescence intensity of the nanofilm. The fluorescence intensity at 410 nm was observed to be proportional to the concentration of viruses in the range of 0.1ā€“10 ng Lāˆ’1 (0.15ā€“15 pg) with the lower calculated detection limit of 2.29 ng Lāˆ’1 (3.4 pg). The intensity of the fluorescence emission is not affected by the thickness of the polythiophene film and the nature of TNV specific binding sites. Kinetic data analyses showed that the nanofilm responds to TNV within 2 min; and cross-selectivity studies with tobacco mosaic virus (TMV) showed an excellent specificity for the targeted TNV. These binding experiments demonstrate the potential of fluorescence emission for the specific, label free and rapid detection of viruses using nanofilm sensors. Taking into account the lower limit of detection, the fluorescence sensing reported here is reliable, simple to perform, rapid, cost-effective and offers a sensitive analytical method for virus detection in water resources.en
dc.exception.ref2021codes254aen
dc.funderCouncil of Scientific and Industrial Reserach (CSIR)en
dc.identifier.citationWankar, S., Turner, N.W., Krupadam, R.J. (2016) Polythiophene nanofilms for sensitive fluorescence detection of viruses in drinking water. Biosensors and Bioelectronics, 82, pp.20ā€“25.en
dc.identifier.doihttps://doi.org/10.1016/j.bios.2016.03.020
dc.identifier.urihttp://oro.open.ac.uk/45651/
dc.identifier.urihttp://hdl.handle.net/2086/16999
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidWM 2A.3.2en
dc.publisherElsevieren
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectVirusen
dc.subjectDrinking Wateren
dc.subjectMIPen
dc.subjectSensoren
dc.titlePolythiophene nanofilms for sensitive fluorescence detection of viruses in drinking wateren
dc.typeArticleen

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
1-s2.0-S0956566316302111-main.pdf
Size:
471.75 KB
Format:
Adobe Portable Document Format
Description:
Mian Article (Open access)
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.2 KB
Format:
Item-specific license agreed upon to submission
Description: