An observational cohort feasibility study to identify microvesicle and miRNA biomarkers of acute kidney injury following paediatric cardiac surgery

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dc.cclicenceCC-BY-NC-NDen
dc.contributor.authorSullo, Nikol
dc.contributor.authorMariani, Silvia
dc.contributor.authorJnTala, Maria
dc.contributor.authorKumar, Tracy
dc.contributor.authorWozniak, Marcin
dc.contributor.authorPais, Paolo
dc.contributor.authorWestrope, Claire
dc.contributor.authorLotto, Attilio
dc.contributor.authorMurphy, Gavin
dc.contributor.authorSmallwood, Dawn T.
dc.date.acceptance2018-09-01
dc.date.accessioned2019-11-12T15:47:39Z
dc.date.available2019-11-12T15:47:39Z
dc.date.issued2018-09
dc.descriptionThe file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.en
dc.description.abstractObjectives: Micro-RNA, small noncoding RNA fragments involved in gene regulation, and microvesicles, membrane-bound particles less than 1 μm known to regulate cellular processes including responses to injury, may serve as disease-specific biomarkers of acute kidney injury. We evaluated the feasibility of measuring these signals as well as other known acute kidney injury biomarkers in a mixed pediatric cardiac surgery population. Design: Single center prospective cohort feasibility study. Setting: PICU. Patients: Twenty-four children (≤ 17 yr) undergoing cardiac surgery with cardiopulmonary bypass without preexisting inflammatory state, acute kidney injury, or extracorporeal life support. Interventions: None. Measurements and Main Results: Acute kidney injury was defined according to modified Kidney Diseases Improving Global Outcomes criteria. Blood and urine samples were collected preoperatively and at 6–12 and 24 hours. Microvesicles derivation was assessed using flow cytometry and NanoSight analysis. Micro-RNAs were isolated from plasma and analyzed by microarray and quantitative real-time polymerase chain reaction. Data completeness for the primary outcomes was 100%. Patients with acute kidney injury (n = 14/24) were younger, underwent longer cardiopulmonary bypass, and required greater inotrope support. Acute kidney injury subjects had different fractional content of platelets and endothelial-derived microvesicles before surgery. Platelets and endothelial microvesicles levels were higher in acute kidney injury patients. A number of micro-RNA species were differentially expressed in acute kidney injury patients. Pathway analysis of candidate target genes in the kidney suggested that the most often affected pathways were phosphatase and tensin homolog and signal transducer and activator of transcription 3 signaling. Conclusions: Microvesicles and micro-RNAs expression patterns in pediatric cardiac surgery patients can be measured in children and potentially serve as tools for stratification of patients at risk of acute kidney injury.en
dc.exception.ref2021codes254aen
dc.funderNIHR (National Institute for Health Research)en
dc.funder.otherBritish Heart Foundationen
dc.funder.otherHeart Linken
dc.identifier.citationSullo, N., Mariani, S., JnTala, M., Kumar, T., Wozniak, M., Smallwood, D., Pais, P., Westrope, C., Lotto, A., Murphy, G. (2018) An observational cohort feasibility study to identify microvesicle and miRNA biomarkers of acute kidney injury following paediatric cardiac surgery. Pediatric Critical Care Medicine,19(9), pp.816–830.en
dc.identifier.doihttps://doi.org/10.1097/pcc.0000000000001604
dc.identifier.urihttps://dora.dmu.ac.uk/handle/2086/18779
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidRG/13/6/29947, CH/12/1/29419, and PG/11/95/29173en
dc.publisherWolters Kluweren
dc.researchinstituteInstitute for Allied Health Sciences Researchen
dc.subjectMicrovesicleen
dc.subjectMicro-RNAen
dc.subjectAcute Kidney Injuryen
dc.subjectPediatric Cardiac Surgeryen
dc.titleAn observational cohort feasibility study to identify microvesicle and miRNA biomarkers of acute kidney injury following paediatric cardiac surgeryen
dc.typeArticleen

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