Enterotoxin Escherichia coli STa activates a nitric oxide-dependent myenteric plexus secretory reflex in the rat ileum.

dc.contributor.authorRolfe, Vivien E.
dc.contributor.authorLevin, Roy J.
dc.date.accessioned2011-11-29T14:13:25Z
dc.date.available2011-11-29T14:13:25Z
dc.date.issued1994
dc.description.abstractMucosally added enterotoxin Escherichia coli STa increased the electrogenic Cl- secretion measured as the short-circuit current (Isc) across isolated muscle-stripped and muscle-unstripped rat mid-ilea incubated in vitro. 2. Pretreatment with serosal L-NAME (N omega-nitro-L-arginine methyl ester) or tetrodotoxin (TTX) significantly reduced the maximum Isc and the duration of action of STa in the unstripped but not stripped ilea. D-NAME (serosal), indomethacin or 5-hydroxy-tryptamine-desensitization was ineffective on STa-induced Isc in either stripped or unstripped ilea. 3. Serosal capsaicin reduced the maximum Isc of STa and its duration of action in unstripped ilea. 4. L-Arginine induced a significantly larger increase in the Isc across unstripped ilea than across stripped ilea; this could be significantly reduced by serosal L-NAME or TTX, although these were ineffective in stripped ilea. 5. Pretreatment of anaesthetized rats with I.P. L-NAME suppressed the fluid secretion induced by luminal STa in ilea in vivo but had no effect on that induced by luminal carbachol. 6. Mucosal STa increased electrogenic Cl- secretion across intact rat ileum in vitro by activating a capsaicin-sensitive, nitric oxide-dependent myenteric plexus-mediated secretory reflex. The suppression by L-NAME of STa induced ileal fluid secretion in vivo probably involves the inhibition of this reflex.en
dc.identifier.citationRolfe, V.E. and Levin, R.J. (1994) Enterotoxin Escherichia coli STa activates a nitric oxide-dependent myenteric plexus secretory reflex in the rat ileum. Journal of Physiology, 475 (3), pp. 531-537.en
dc.identifier.doihttps://doi.org/10.1113/jphysiol.1994.sp020091
dc.identifier.urihttp://hdl.handle.net/2086/5419
dc.language.isoenen
dc.peerreviewedYesen
dc.publisherJournal of Physiologyen
dc.researchgroupBiomedical and Environmental Health
dc.subjectNitric oxideen
dc.subjectintestinal secretionen
dc.subjectdiarrhoeal diseaseen
dc.subjectEscherichia colien
dc.subjectmyenteric plexusen
dc.titleEnterotoxin Escherichia coli STa activates a nitric oxide-dependent myenteric plexus secretory reflex in the rat ileum.en
dc.typeArticleen

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