Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models

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dc.cclicenceCC-BYen
dc.contributor.authorPercival, B.C.en
dc.contributor.authorGrootveld, Martinen
dc.contributor.authorGibson, M.en
dc.contributor.authorOsman, Y.en
dc.contributor.authorMolinari, M.en
dc.contributor.authorJafari, F.en
dc.contributor.authorSahota, T. S.en
dc.contributor.authorMartin, M.en
dc.contributor.authorCasanova, F.en
dc.contributor.authorMather, M.L.en
dc.contributor.authorEdgar, M.en
dc.contributor.authorMasania, J.en
dc.contributor.authorWilson, Philippe B.en
dc.date.acceptance2018-12-13en
dc.date.accessioned2019-01-09T11:04:06Z
dc.date.available2019-01-09T11:04:06Z
dc.date.issued2018-12-27
dc.descriptionopen access articleen
dc.description.abstractNovel sensing technologies for liquid biopsies offer promising prospects for the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognise unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimised experiments, including α-glucose (≤2.8 mmol/L) and acetone (25 µmol/L), and additionally in readily accessible biofluids, particularly human urine. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics applications, and offer a perspective on the future of this technique appealing to ‘point-of-care’ applications.en
dc.exception.reasonThe output was published as gold open accessen
dc.funderN/Aen
dc.identifier.citationPercival, B.C., Grootveld, M., Gibson, M., Osman, Y., Molinari, M., Jafari, F., Sahota, T., Martin, M., Casanova, F., Mather, M.L., Edgar, M., Masania, J., Wilson, Philippe B. (2019) Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models. High-Throughput, 8(1), 2.en
dc.identifier.doihttps://doi.org/10.3390/ht8010002
dc.identifier.urihttp://hdl.handle.net/2086/17414
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidN/Aen
dc.publisherMDPIen
dc.researchgroupChemistry for Healthen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectmetabolomicsen
dc.subjectbenchtop 60 MHz NMR analysisen
dc.subjectbiomarkersen
dc.subjectbiomoleculesen
dc.subjectvalidationen
dc.subjectprotocolen
dc.subjectdiabetesen
dc.titleLow-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Modelsen
dc.typeArticleen

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