Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples

Date

2017-10-26

Advisors

Journal Title

Journal ISSN

ISSN

Volume Title

Publisher

Multidisciplinary Digital Publishing Institute

Type

Article

Peer reviewed

Yes

Abstract

Liriope and Ophiopogon species have a long history of use as traditional medicines across East Asia. They have also become widely used around the world for ornamental and landscaping purposes. The morphological similarities between Liriope and Ophiopogon taxa have made the taxonomy of the two genera problematic and caused confusion about the identification of individual specimens. Molecular approaches could be a useful tool for the discrimination of these two genera in combination with traditional methods. Seventy-five Liriope and Ophiopogon samples from the UK National Plant Collections of Ophiopogon and Liriope were analyzed. The 5′ end of the DNA barcode region of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcLa) was used for the discrimination of the two genera. A single nucleotide polymorphism (SNP) between the two genera allowed the development of discriminatory tests for genus-level identification based on specific PCR and high-resolution melt curve (HRM) assays. The study highlights the advantage of incorporating DNA barcoding methods into plant identification protocols and provides simple assays that could be used for the quality assurance of commercially traded plants and herbal drugs.

Description

open access article

Keywords

Ophiopogon, Liriope, rbcL, DNA barcoding, high-resolution melt curve (HRM) analysis

Citation

Masiero, E. et al. (2017) Genus-Specific Real-Time PCR and HRM Assays to Distinguish Liriope from Ophiopogon Samples. Plants 2017, 6 (4), 53

Rights

Research Institute

Institute for Allied Health Sciences Research