Peroxidase-catalysed coloration of wool fabrics

An enzyme-based textile coloration process using peroxidase (EC1.11.1.7) was investigated for its potential as an alternative to conventional textile dyeing processes, with the benefits of being low in energy use and non-damaging to fibres. The current study presents a process for the coloration of wool fabric using peroxidase oxidation of a range of different aromatic compounds in the presence of hydrogen peroxide. The results revealed that wool can be successfully dyed by peroxidase-catalysed coloration at temperatures as low as 30°C. By controlling the pH values and buffer systems during processing, a diverse colour palette was produced, depending on the small molecular aromatic compound used as the precursor. Colour fastness testing found that fastness to washing, rubbing and light properties achieved good to excellent ratings, with further improvement to wash fastness provided by a post-soaping wash. No fibre damage occurred due to peroxidase-catalysed coloration. This enzyme coloration process is a promising alternative to conventional wool dyeing processes with the advantage of effective dyeing at low temperatures, therefore having the potential of reducing energy consumption and preventing fibre damage.