Comparison of presumptive blood test kits including Hexagon OBTI

Date

2008-05-07

Advisors

Journal Title

Journal ISSN

ISSN

Volume Title

Publisher

Wiley Blackwell

Type

Article

Peer reviewed

Yes

Abstract

Four presumptive blood tests, Hexagon OBTI, Hemastix , Leucomalachite green (LMG), and Kastle-Meyer (KM) were compared for their sensitivity in the identification of dried bloodstains. Stains of varying blood dilutions were subjected to each presumptive test and the results compared. The Hexagon OBTI buffer volume was also reduced to ascertain whether this increased the sensitivity of the kit. The study found that Hemastix was the most sensitive test for trace blood detection. Only with the reduced buffer volume was the Hexagon OBTI kit as sensitive as the LMG and KM tests. However, the Hexagon OBTI kit has the advantage of being a primate specific blood detection kit. This study also investigated whether the OBTI buffer within the kit could be utilized for DNA profiling after presumptive testing. The results show that DNA profiles can be obtained from the Hexagon OBTI kit buffer directly.

Description

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.

Keywords

forensic science, presumptive blood testing, Hexagon OBTI, Kastle-Meyer, Leucomalachite green, Hemastix

Citation

Johnston, E., Ames, C.E., Dagnall, K.E., Foster, J., Daniel, B.E. (2008) Comparison of presumtive blood test kits including Hexagon OBTI. Journal of Forensic Sciences, 53 (3), pp. 687-689

Rights

Research Institute

Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)