Hybrid Aptamer-Molecularly Imprinted Polymer (aptaMIP) Nanoparticles from Protein Recognition—A Trypsin Model

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dc.cclicenceCC-BY-NCen
dc.contributor.authorSullivan, Mark
dc.contributor.authorClay, Oliver
dc.contributor.authorMoazami, Michael
dc.contributor.authorWatts, Jonathan
dc.contributor.authorTurner, Nicholas W.
dc.date.acceptance2021-03-24
dc.date.accessioned2021-06-15T11:08:12Z
dc.date.available2021-06-15T11:08:12Z
dc.date.issued2021-03-24
dc.descriptionopen access articleen
dc.description.abstractAptamers offer excellent potential for replacing antibodies for molecular recognition purposes however their performance can compromise with biological/environmental degradation being a particular problem. Molecularly imprinted polymers (MIPs) offer an alternative to biological materials and while these offer the robustness and ability to work in extreme environmental conditions, they often lack the same recognition performance. By slightly adapting the chemical structure of a DNA aptamer we have incorporated it for use as the recognition part of a MIP, thus creating an aptamer-MIP hybrid or aptaMIP. Here we have developed these for the detection of the target protein trypsin. The aptaMIP nanoparticles offer superior binding affinity over conventional MIP nanoparticles (nanoMIPs), with KD values of 6.8 × 10-9 (± 0.2 × 10-9) M and 12.3 × 10-9 (± 0.4 × 10-9) M for the aptaMIP and nanoMIP, respectively. The aptaMIP also outperforms the aptamer only (10.3 x 10-9 M). Good selectivity against other protein targets is observed. Using Surface Plasmon Resonance, the limit of detection for aptaMIP nanoparticles was two-fold lower (2nM) compared to the nanoMIP (4 nM). Introduction of the aptamer as a “macro-monomer” into the MIP scaffold has beneficial effects and offers potential to improve this class of polymers significantly.en
dc.funderEPSRC (Engineering and Physical Sciences Research Council)en
dc.identifier.citationSullivan, M. V., Clay, O., Moazami, M. P., Watts, J. K., Turner, N. W. (2021) Hybrid Aptamer-Molecularly Imprinted Polymer (aptaMIP) Nanoparticles from Protein Recognition—A Trypsin Model. Macromolecular Bioscience, 21(5), 2100002.en
dc.identifier.doihttps://doi.org/10.1002/mabi.202100002
dc.identifier.issn1616-5187
dc.identifier.urihttps://dora.dmu.ac.uk/handle/2086/20996
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidEP/S003339/1)en
dc.publisherWileyen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectaptameren
dc.subjectmolecularly imprinted polymersen
dc.subjectnanoparticlesen
dc.subjectproteinen
dc.titleHybrid Aptamer-Molecularly Imprinted Polymer (aptaMIP) Nanoparticles from Protein Recognition—A Trypsin Modelen
dc.typeArticleen

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