Physicomechanical properties of sintered scaffolds formed from porous and protein-loaded poly (DL-lactic-co-glycolic acid) microspheres for potential use in bone tissue engineering

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dc.cclicenceN/Aen
dc.contributor.authorBoukari, Y.
dc.contributor.authorScurr, D.J.
dc.contributor.authorQutachi, Omar
dc.contributor.authorMorris, A.P.
dc.contributor.authorDoughty, S.W.
dc.contributor.authorRahman, C.V.
dc.contributor.authorBilla, N.
dc.date.acceptance2015-06-02
dc.date.accessioned2019-05-14T13:23:21Z
dc.date.available2019-05-14T13:23:21Z
dc.date.issued2015-07-02
dc.descriptionThe Publisher's final version can be found by following the DOI link.en
dc.description.abstractAn injectable poly(DL-lactic-co-glycolic acid) (PLGA) system comprising both porous and protein-loaded microspheres capable of forming porous scaffolds at body temperature was developed for tissue regeneration purposes. Porous and non-porous (lysozyme loaded) PLGA microspheres were formulated to represent ‘low molecular weight’ 22–34 kDa, ‘intermediate molecular weight’ (IMW) 53 kDa and ‘high molecular weight’ 84–109 kDa PLGA microspheres. The respective average size of the microspheres was directly related to the polymer molecular weight. An initial burst release of lysozyme was observed from both microspheres and scaffolds on day 1. In the case of the lysozyme-loaded microspheres, this burst release was inversely related to the polymer molecular weight. Similarly, scaffolds loaded with 1 mg lysozyme/g of scaffold exhibited an inverse release relationship with polymer molecular weight. The burst release was highest amongst IMW scaffolds loaded with 2 and 3 mg/g. Sustained lysozyme release was observed after day 1 over 50 days (microspheres) and 30 days (scaffolds). The compressive strengths of the scaffolds were found to be inversely proportional to PLGA molecular weight at each lysozyme loading. Surface analysis indicated that some of the loaded lysozyme was distributed on the surfaces of the microspheres and thus responsible for the burst release observed. Overall the data demonstrates the potential of the scaffolds for use in tissue regeneration.en
dc.funderNo external funderen
dc.identifier.citationBoukari, Y., Scurr, D.J., Qutachi, O., Morris, A.P., Doughty, S.W., Rahman, C.V., Billa, N. (2015) Physicomechanical properties of sintered scaffolds formed from porous and protein-loaded poly (DL-lactic-co-glycolic acid) microspheres for potential use in bone tissue engineering. Journal of Biomaterials Science (Polymer edition), 26 (12), pp. 796-811en
dc.identifier.doihttps://doi.org/10.1080/09205063.2015.1058696
dc.identifier.urihttps://www.dora.dmu.ac.uk/handle/2086/17817
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidEUFP7-NMP.20102.3-1en
dc.publisherTaylor and Francisen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectmicrosphereen
dc.subjectscaffolden
dc.subjectlysozymeen
dc.subjectPLGAen
dc.subjectporousen
dc.subjecttissue engineeringen
dc.subjectBMP-2en
dc.subjectinjectableen
dc.titlePhysicomechanical properties of sintered scaffolds formed from porous and protein-loaded poly (DL-lactic-co-glycolic acid) microspheres for potential use in bone tissue engineeringen
dc.typeArticleen

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