Occurrence of ESBL-Producing Escherichia coli ST131, Including the H30-Rx and C1-M27 Subclones, Among Urban Seagulls from the United Kingdom
| dc.cclicence | CC-BY-NC | en |
| dc.contributor.author | Zendri, Flavia | |
| dc.contributor.author | Maciuca, Iuliana E. | |
| dc.contributor.author | Moon, Simon | |
| dc.contributor.author | Jones, Phillip H. | |
| dc.contributor.author | Wattret, Andy | |
| dc.contributor.author | Jenkins, R. O. | |
| dc.contributor.author | Baxter, Andy | |
| dc.contributor.author | Timofte, Dorina | |
| dc.date.acceptance | 2019-10-10 | |
| dc.date.accessioned | 2019-12-18T08:29:05Z | |
| dc.date.available | 2019-12-18T08:29:05Z | |
| dc.date.issued | 2019-12-12 | |
| dc.description | The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. | en |
| dc.description.abstract | Antimicrobial resistance is a public health concern. Understanding any role that urban seagulls may have as a reservoir of resistant bacteria could be important for reducing transmission. This study investigated fecal Escherichia coli isolates from seagulls (herring gulls and lesser black-backed gulls) to determine the prevalence of extended-spectrum cephalosporin-resistant (ESC-R) and fluoroquinolone-resistant E. coli among gull species from two cities (Taunton and Birmingham) in the United Kingdom (UK). We characterized the genetic background and carriage of plasmid-mediated resistance genes in extended-spectrum β-lactamase (ESBL)-producing E. coli obtained from these birds. Sixty ESC-R E. coli isolates were obtained from 39 seagulls (39/78, 50%), of which 28 (28/60, 46.7%) were positive for plasmid-mediated CTX-M and/or AmpC β-lactamase resistance genes. Among these, blaCTX-M-15, blaCTX-M-14, and blaCMY-2 predominated. Three isolates belonging to the B2-ST131 clone were detected, of which two harbored blaCTX-M-15 (typed to C2/H30Rx) and one harbored blaCTX-M-27 and was typed to C1/H30-R (recently described as the C1-M27 sublineage). The plasmid-mediated quinolone resistance (PMQR) gene carriage prevalence (11.7%) consisted of aac(6′)-Ib-cr and qnrB genes. No carbapenem or colistin resistance genes were detected. Urban seagulls in the UK are colonized and can spread major antimicrobial-resistant E. coli isolates harboring ESBL and PMQR determinants, including clinically important strains such as the pandemic clone B2-ST131 and the C1-M27 subclade. This is the first report of ST131-C1-M27 subclade in wildlife in the UK and in seagulls worldwide. | en |
| dc.funder | No external funder | en |
| dc.identifier.citation | Zendri, F.,Maciuca, I.E., Moon, S., Jones, P.H., Wattret, A., Jenkins, R., Baxter, A. and Timoft, D. (2019) Occurrence of ESBL-producing Escherichia coli ST131 including the H30-Rx and C1-M27 subclones among urban seagulls from United Kingdom. Microbial Drug Resistance, | en |
| dc.identifier.doi | https://doi.org/10.1089/mdr.2019.0351 | |
| dc.identifier.issn | 1076-6294 | |
| dc.identifier.uri | https://dora.dmu.ac.uk/handle/2086/18962 | |
| dc.language.iso | en | en |
| dc.peerreviewed | Yes | en |
| dc.projectid | N/A | en |
| dc.publisher | Mary Ann Liebert, Inc. | en |
| dc.researchinstitute | Institute for Allied Health Sciences Research | en |
| dc.subject | Escherichia coli | en |
| dc.subject | B2-ST131 | en |
| dc.subject | Larus spp. | en |
| dc.subject | ESBLs | en |
| dc.subject | AmpC | en |
| dc.subject | PMQR | en |
| dc.subject | C1-M27 | en |
| dc.title | Occurrence of ESBL-Producing Escherichia coli ST131, Including the H30-Rx and C1-M27 Subclones, Among Urban Seagulls from the United Kingdom | en |
| dc.type | Article | en |