Synthesis and Identification of FITC-Insulin Conjugates Produced Using Human Insulin and Insulin Analogues for Biomedical Applications

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dc.contributor.authorJacob, Dollyen
dc.contributor.authorTaylor, M. Joanen
dc.contributor.authorTomlins, P.en
dc.contributor.authorSahota, T. S.en
dc.date.accessioned2016-01-04T11:05:43Z
dc.date.available2016-01-04T11:05:43Z
dc.date.issued2015
dc.descriptionWe thank EPSRC National Mass Spectrometry Facility, Swansea University for their help with FITC-insulin analysis.en
dc.description.abstractfluorescein isothiocyanate (FITC) and the conjugate species produced were identified using high performance liquid chromatography and electrospray mass spectroscopy. Mono-labelled FITC-insulin conjugate (A1 or B1) was successfully produced using human insulin at short reaction times (up to 5 h) however the product always contained some unlabelled native human insulin. As the reaction time was increased over 45 h, no unlabelled native human insulin was present and more di-labelled FITC-insulin conjugate (A1B1) was produced than mono-labelled conjugate with the appearance of tri-labelled conjugate (A1B1B29) after 20 h reaction time. The quantities switch from mono-labelled to di-labelled FITC-insulin conjugate between reaction times 9 and 20 h. In the presence of phenol or m-cresol, there appears to be a 10 % decrease in the amount of mono-labelled conjugate and an increase in di-labelled conjugate produced at lower reaction times. Clinically used insulin analogues present in commercially available preparations were successfully fluorescently labelled for future biomedical applications.en
dc.explorer.multimediaNoen
dc.funderResearch studentship for D Jacob by De Montfort Universityen
dc.identifier.citationJacob, D. et al. (2015) Synthesis and Identification of FITC-Insulin Conjugates Produced Using Human Insulin and Insulin Analogues for Biomedical Applications. Journal of Fluorescence, DOI 10.1007/s10895-015-1748-1en
dc.identifier.doihttps://doi.org/10.1007/s10895-015-1748-1
dc.identifier.urihttp://hdl.handle.net/2086/11426
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidInSmarten
dc.publisherSpringeren
dc.researchgroupPharmaceutical Technologiesen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectInsulinen
dc.subjectFluorescent labellingen
dc.subjectFITC-insulinen
dc.subjectHPLCen
dc.subjectMono-labelleden
dc.titleSynthesis and Identification of FITC-Insulin Conjugates Produced Using Human Insulin and Insulin Analogues for Biomedical Applicationsen
dc.typeArticleen

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