Structural basis of mannan-binding lectin recognition by its associated serine protease MASP-1: implications for complement activation

Date

2011-11

Advisors

Journal Title

Journal ISSN

ISSN

Volume Title

Publisher

Science Direct

Type

Article

Peer reviewed

Yes

Abstract

Complement activation contributes directly to health and disease. It neutralizes pathogens and stimulates immune processes. Defects lead to immunodeficiency and autoimmune diseases, whereas inappropriate activation causes self-damage. In the lectin and classical pathways, complement is triggered upon recognition of a pathogen by an activating complex. Here we present the first structure of such a complex in the form of the collagen-like domain of mannan-binding lectin (MBL) and the binding domain of its associated protease (MASP-1/-3). The collagen binds within a groove using a pivotal lysine side chain that interacts with Ca(2+)-coordinating residues, revealing the essential role of Ca(2+). This mode of binding is prototypic for all activating complexes of the lectin and classical pathways, and suggests a general mechanism for the global changes that drive activation. The structural insights reveal a new focus for inhibitors and we have validated this concept by targeting the binding pocket of the MASP.

Description

Keywords

MASP-1, complement activation

Citation

Gingras, A.R., Venkatraman Girija, U., Keeble, A.H., Panchal, R., Mitchell, D.A., Moody, P.C.E. and Wallis R. (2011) Structural basis of mannan-binding lectin recognition by its associated serine protease MASP-1: implications for complement activation. Structure, 19 (11), pp. 1635-1643

Rights

Research Institute

Institute for Allied Health Sciences Research