Green synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobin

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dc.cclicenceCC-BY-NC-NDen
dc.contributor.authorSullivan, Mark Vincent
dc.contributor.authorStockburn, William J.
dc.contributor.authorHawes, Philippa C.
dc.contributor.authorMercer, Tim
dc.contributor.authorReddy, Subrayal M.
dc.date.acceptance2020-12-10
dc.date.accessioned2021-07-17T13:39:50Z
dc.date.available2021-07-17T13:39:50Z
dc.date.issued2020-12-10
dc.descriptionopen access articleen
dc.description.abstractWe have developed a low-cost molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify myoglobin in a biological sample. The assay uses a previously unreported method for the development of microwave-assisted rapid synthesis of aldehyde functionalized magnetic nanoparticles, in just 20 min. The aldehyde functionalized nanoparticles have an average size of 7.5 nm ± 1.8 and saturation magnetizations of 31.8 emu g−1 with near-closed magnetization loops, confirming their superparamagnetic properties. We have subsequently shown that protein tethering was possible to the aldehyde particles, with 0.25 ± 0.013 mg of myoglobin adsorbed to 20 mg of the nanomaterial. Myoglobin-specific fluorescently tagged MIP (F-MIP) particles were synthesized and used within the assay to capture myoglobin from a test sample. Excess F-MIP was removed from the sample using protein functionalized magnetic nanoparticles (Mb-SPION), with the remaining sample analyzed using fluorescence spectroscopy. The obtained calibration plot of myoglobin showed a linear correlation ranging from 60 pg ml−1 to 6 mg ml−1 with the limit of detection of 60 pg ml−1. This method was successfully used to detect myoglobin in spiked fetal calf serum, with a recovery rate of more than 93%.en
dc.funderWellcome Trusten
dc.identifier.citationSullivan, M.V., Stockburn, W.J., Hawes, P.C., Mercer,T. and Reddy, S.M. (2021) Green synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobin. Nanotechnology, 32(9), 095502.en
dc.identifier.doihttps://doi.org/10.1088/1361-6528/abce2d
dc.identifier.issn0957-4484
dc.identifier.urihttps://dora.dmu.ac.uk/handle/2086/21109
dc.language.isoenen
dc.projectid108003/A/15/Zen
dc.publisherIOP Publishingen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectMolecularly Imprinted Polymersen
dc.subjectProtein Recognitionen
dc.subjectMagnetic Nanoparticlesen
dc.titleGreen synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobinen
dc.typeArticleen

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