Dried blood spot sampling with LC-MS analysis for routine therapeutic caffeine monitoring in neonates

Date

2012-11

Advisors

Journal Title

Journal ISSN

ISSN

Volume Title

Publisher

International Scholarly Research Network

Type

Article

Peer reviewed

Yes

Abstract

A liquid chromatography–mass spectrometry (LC-MS) method was developed and validated for the determination of therapeutic levels of caffeine in dried blood spot (DBS) samples. Caffeine is used in the treatment of Apnoea of Prematurity (AoP) in newborn children. Calibration DBS samples were prepared by spotting 15l of whole blood spiked with the analyte onto specimen collection cards. 5mm disks cut from the centre of the DBS were extracted in methanol containing the internal standard. The extract was separated using a Zorbax Eclipse Plus C18 column and the MS, operated in electrospray positive ion mode, used single ion monitoring at m/z 195 for caffeine and 198 for the IS. The overall extraction recovery of caffeine from spiked blood spots was demonstrated to be 44-47%. Validation of the micro-analytical method showed good precision (coefficient of variation) and accuracy (relative error) and specificity and was linear within the tested calibration range 500-25000ng/ml for caffeine. Investigation of different specimen collection papers revealed different matrix effects with significant ion suppression from the FTA Elute paper itself. Requiring only a micro volume (15µl) blood sample for analysis, the developed DBS based micro-analytical method has the potential to facilitate the routine monitoring of caffeine in neonates.

Description

Keywords

dried blood spot (DBS), caffeine, LC-MS, Guthrie card, therapeutic drug monitoring, bioanalysis, neonates

Citation

Lawson, G. et al. (2012) Dried Blood Spot Sampling with LC-MS Analysis for Routine Therapeutic Caffeine Monitoring in Neonates. ISRN Chromatography, 2012, Article ID 828719

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Research Institute

Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)