Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide

Sobolev, V. E.; Jenkins, R. O.; Goncharov, Nikolay V.

Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide

dc.cclicence	CC-BY-NC-ND	en
dc.contributor.author	Sobolev, V. E.	en
dc.contributor.author	Jenkins, R. O.	en
dc.contributor.author	Goncharov, Nikolay V.	en
dc.date.acceptance	2017-02-23	en
dc.date.accessioned	2017-03-08T16:16:43Z
dc.date.available	2017-03-08T16:16:43Z
dc.date.issued	2017-03-01
dc.description	The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.	en
dc.description.abstract	Glycosaminoglycans (GAGs) in the urine of Wistar rats, and on the surface of the epithelium and lamina propria of the bladder, were quantitatively assessed before and after acute intoxication with paraoxon or cyclophosphamide. Paraoxon was administered subcutaneously (s.c.) twice with an interval of 1 hour to a final dose of 275 mg/kg; cyclophosphamide was administered intraperitoneally (i.p.) with a single dose of 100 mg/kg or to a final dose of 240 mg/kg (three times per 80 mg/kg every 12 hours). GAGs on the surface of the epithelium and lamina propria of the urinary bladder of rats were quantitatively determined by Alcian blue dye staining. GAGs in the urine were determined spectrophotometrically with 1-9-dimethyl methylene blue. By 48-96 hours after intoxication with either paraoxon or cyclophosphamide, statistically significant increases in the concentration of GAGs were obtained both for the tissues of the bladder and the urine of rats. Cyclophosphamide, in contrast to paraoxon, caused the development of hemorrhagic cystitis in the animals. The synthesis of GAGs is considered to be compensatory response to the toxic xenobiotics.	en
dc.funder	Russian Science Foundation	en
dc.identifier.citation	Sobolev, V. E., Jenkins, R.O. and Goncharov, N. (2017) Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide. Experimental and Toxicologic Pathology, 69 (6), pp. 339-347	en
dc.identifier.doi	https://doi.org/10.1016/j.etp.2017.02.007
dc.identifier.issn	0940-2993
dc.identifier.uri	http://hdl.handle.net/2086/13482
dc.language.iso	en	en
dc.peerreviewed	Yes	en
dc.projectid	16-15-00199	en
dc.publisher	Elsevier	en
dc.relation.ispartofseries	Experimental and Toxicologic Pathology;
dc.researchgroup	Biomedical and Environmental Health	en
dc.researchinstitute	Institute for Allied Health Sciences Research	en
dc.subject	glycosaminoglycans	en
dc.subject	paraoxon	en
dc.subject	cyclophosphamide	en
dc.subject	urinary bladder	en
dc.subject	cystitis	en
dc.title	Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide	en
dc.type	Article	en