Quantitative LC-HRMS analysis of dried blood spots to assess adherence to cardiovascular pharmacotherapy

Date

2017-01-25

Advisors

Journal Title

Journal ISSN

ISSN

DOI

Volume Title

Publisher

The Association for Mass Spectrometry: Applications to the Clinical Lab

Type

Conference

Peer reviewed

Yes

Abstract

The analysis of dried blood spot samples (DBS) by liquid chromatography – high resolution mass spectrometry (LC-HRMS) for assessing adherence to candidate cardiovascular therapeutic drugs was investigated. To evaluate the method 8 mm discs were punched from each DBS and extracted followed by subjecting to LC-HRMS analysis. Trials using the top 11 UK prescribed cardiovascular drugs are reported demonstrating the ability of the system to detect the target analytes during the 24 hour repeat prescription cycle. The system responded successfully to challenges by samples from volunteers of known adherence or receiving no medication. Examples of non-adherence to different medications were identified.

Description

Keywords

dried blood spots, quantitative analysis, LC-HRMS, Adherence

Citation

Lawson, G., Bernieh, D. and Tanna S (2017) Quantitative LC-HRMS analysis of dried blood spots to assess adherence to cardiovascular pharmacotherapy. Mass Spectrometry: Applications to the Clinical Lab (MSACL) 2017. Palm Springs, USA. 22-26 January 2017

Rights

Research Institute

Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)