Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs)

Date

2014-09-12

Advisors

Journal Title

Journal ISSN

ISSN

0024-9297

Volume Title

Publisher

ACS Publications

Type

Article

Peer reviewed

Yes

Abstract

MIP NPs represent one of the current most suitable alternatives to antibodies for molecular recognition and diagnostic applications. Here we present the synthesis of MIP NPs imprinted for 2′-deoxyadenosine (dA) prepared using for the first time a modified polymerizable 2′-deoxyuridine complementary residue. We demonstrate that the introduction of this modified monomer results in an increase of the affinity of the produced MIP NPs, without altering their physical properties such as size, shape, or dispersibility. The imprinted NPs have shown the ability to specifically recognize dA, whereas no imprinting effect was observed for 2′-deoxyguanosine (dG) or deoxycytidine (dC). The results suggest that such monomers (and their phosphoramidites) could be used in the synthesis of oligomer or longer DNA sequences for potentially producing hybrid MIP–DNA materials with improved recognition performance.

Description

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.

Keywords

Nanoparticles, Molecular Imprinting, Apta-MIP

Citation

Poma, A., Brahmbhatt, H., Watts, J.K., Turner, N.W. (2014) Nucleoside-tailored molecularly imprinted polymeric nanoparticles (MIP NPs). Macromolecules, 47(18), pp.6322–6330.

Rights

Research Institute

Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)