Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae)

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dc.cclicenceCC-BY-NCen
dc.contributor.authorMishra, Pryankaen
dc.contributor.authorKumar, Amiten
dc.contributor.authorSivaraman, Gokulen
dc.contributor.authorShukla, Ashutoshen
dc.contributor.authorKaliamoorthy, Ravikumaren
dc.contributor.authorSlater, A.en
dc.contributor.authorVelusamy, Sundaresanen
dc.date.acceptance2017-10-09en
dc.date.accessioned2017-11-15T09:59:29Z
dc.date.available2017-11-15T09:59:29Z
dc.date.issued2017-11-02
dc.descriptionopen access articleen
dc.description.abstractThe steno-endemic species of genus Decalepis are highly threatened by destructive wild harvesting. The medicinally important fleshy tuberous roots of Decalepis hamiltonii are traded as substitute, to meet the international market demand of Hemidesmus indicus. In addition, the tuberous roots of all three species of Decalepis possess similar exudates and texture, which challenges the ability of conventional techniques alone to perform accurate species authentication. This study was undertaken to generate DNA barcodes that could be utilized in monitoring and curtailing the illegal trade of these endangered species. The DNA barcode reference library was developed in BOLD database platform for candidate barcodes rbcL, matK, psbA-trnH, ITS and ITS2. The average intra-specific variations (0–0.27%) were less than the distance to nearest neighbour (0.4–11.67%) with matK and ITS. Anchoring the coding region rbcL in multigene tiered approach, the combination rbcL + matK + ITS yielded 100% species resolution, using the least number of loci combinations either with PAUP or BLOG methods to support a character-based approach. Species-specific SNP position (230 bp) in the matK region that is characteristic of D. hamiltonii could be used to design specific assays, enhancing its applicability for direct use in CITES enforcement for distinguishing it from H. indicus.en
dc.exception.reasonopen access articleen
dc.funderCouncil of Scientific and Industrial Research (CSIR), New Delhi, Indiaen
dc.identifier.citationMishra, P., Kumar, A., Sivaraman, G., Shukla, A.K., Kaliamoorthy, R., Slater, A. and Velusamy, S. (2017) Character-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae). Scientific Reports, 7, 14910en
dc.identifier.doihttps://doi.org/10.1038/s41598-017-14887-8
dc.identifier.urihttp://hdl.handle.net/2086/14862
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidFYP project Biopros-PR (BSC-0106)en
dc.projectidChemBio (BSC-0203)en
dc.publisherNature Publishing Groupen
dc.researchgroupBiomolecular Technology Groupen
dc.subjectAuthenticationen
dc.subjectbiodiversityen
dc.subjectmedicinal plantsen
dc.subjectDNA barcodingen
dc.titleCharacter-based DNA barcoding for authentication and conservation of IUCN Red listed threatened species of genus Decalepis (Apocynaceae)en
dc.typeArticleen

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