Show simple item record

dc.contributor.authorArroo, Randolph R.J.
dc.contributor.authorBhambra, Avninder S.
dc.contributor.authorHano, Christophe
dc.contributor.authorRenda, Gülin
dc.contributor.authorRuparelia, Ketan C.
dc.contributor.authorWang, Meng F
dc.date.accessioned2020-10-05T15:07:43Z
dc.date.available2020-10-05T15:07:43Z
dc.date.issued2020-07-24
dc.identifier.citationArroo, R.R.J., Bhambra, A.S., Hano, C., Renda, G., Ruparelia, K.C., Wang, M.F. (2020) Analysis of plant secondary metabolism using stable isotope‐labelled precursors. Phytochemical Analysis.en
dc.identifier.urihttps://dora.dmu.ac.uk/handle/2086/20238
dc.descriptionSpecial issue of Phytochemical Analysis on NMR-based analytical techniques. open access articleen
dc.description.abstractIntroduction Analysis of biochemical pathways typically involves feeding a labelled precursor to an organism, and then monitoring the metabolic fate of the label. Initial studies used radioisotopes as a label and then monitored radioactivity in the metabolic products. As analytical equipment improved and became more widely available, preference shifted the use stable ‘heavy’ isotopes like deuterium (2H)‐, carbon‐13 (13C)‐ and nitrogen‐15 (15N)‐atoms as labels. Incorporation of the labels could be monitored by mass spectrometry (MS), as part of a hyphenated tool kits, e.g. Liquid chromatography (LC)–MS, gas chromatography (GC)–MS, LC–MS/MS. MS offers great sensitivity but the exact location of an isotope label in a given metabolite cannot always be unambiguously established. Nuclear magnetic resonance (NMR) can also be used to pick up signals of stable isotopes, and can give information on the precise location of incorporated label in the metabolites. However, the detection limit for NMR is quite a bit higher than that for MS. Objectives A number of experiments involving feeding stable isotope‐labelled precursors followed by NMR analysis of the metabolites is presented. The aim is to highlight the use of NMR analysis in identifying the precise fate of isotope labels after precursor feeding experiments. As more powerful NMR equipment becomes available, applications as described in this review may become more commonplace in pathway analysis. Conclusion and Prospects NMR is a widely accepted tool for chemical structure elucidation and is now increasingly used in metabolomic studies. In addition, NMR, combined with stable isotope feeding, should be considered as a tool for metabolic flux analyses.en
dc.language.isoenen
dc.publisherWileyen
dc.subjectBiosynthesisen
dc.subjectBiosynthetic pathwaysen
dc.subjectMetabolic fluxen
dc.subjectNMRen
dc.subjectTerpenoidsen
dc.subjectTropane alkaloidsen
dc.titleAnalysis of plant secondary metabolism using stable isotope‐labelled precursorsen
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.1002/pca.2955
dc.peerreviewedYesen
dc.funderNo external funderen
dc.cclicenceCC BYen
dc.date.acceptance2020-05-07
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record