Flavonols with a catechol or pyrogallol substitution pattern on ring B readily form stable dimers in phosphate buffered saline at four degrees Celsius.
Phosphate buffered saline (PBS) is a buffer commonly used in biological research. The stability of a series of flavonoids, i.e. myricetin, kaempferol, baicalein, luteolin and quercetin in PBS was assessed. Apigenin proved very stable when incubated with PBS and was used as a control. Kaempferol and baicalein were less stable, and small amounts of oxidized and hydroxylated products could be detected. Flavonols with catechol or pyrogallol structure in ring B are unstable in PBS (pH=7.4) at 4 ºC and were converted into their stable dimers and oxidized products within 5 seconds. The chosen experimental conditions improved the stability of dimers and allowed their detection.
Collaboration between: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou China; Institut für Pharmazie und Lebensmittelchemie, Universität Würzburg, Würzburg, Germany; De Montfort University – Leicester School of Pharmacy, Leicester, UK; Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.
Citation : Cao, H., Högger, P., Arroo, R., Xiao, J. (2020) Flavonols with a catechol or pyrogallol substitution pattern on ring B readily form stable dimers in phosphate buffered saline at four degrees Celsius. Food Chemistry, 311, 125902
ISSN : 0308-8146
Research Institute : Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)
Peer Reviewed : Yes
- Leicester School of Pharmacy