dc.contributor.author | Jacob, Dolly | |
dc.contributor.author | Tomlins, Paul | |
dc.contributor.author | Taylor, M. Joan | |
dc.contributor.author | Sahota, T. S. | |
dc.date.accessioned | 2019-11-12T11:31:11Z | |
dc.date.available | 2019-11-12T11:31:11Z | |
dc.date.issued | 2019-11-09 | |
dc.identifier.citation | Jacob, D., Tomlins, P., Sahota, T.S., Taylor, M.J. (2019) Insulin Solution Stability and Biocompatibility with Materials Used for an Implantable Insulin Delivery Device Using Reverse Phase HPLC Methods. Applied Sciences, 9, 4794. | en |
dc.identifier.uri | https://dora.dmu.ac.uk/handle/2086/18772 | |
dc.description | open access article | en |
dc.description.abstract | Abstract: Insulin (Humulin® R IU500) has been delivered from an implantable artificial pancreas in
diabetic rats and pigs. The artificial pancreas which was implanted in the peritoneum was fabricated
from several biocompatible materials such as polycarbonate, stainless steel, polyurethane, titanium
and a polyurethane resin. The device also contains a glucose responsive smart gel which controls the
di usion of insulin dependent on the surrounding glucose environment. As the insulin reservoir is
refillable and in contact with the device materials, assessing its biocompatibility with these various
device component materials was conducted. Insulin can undergo chemical degradation mainly
via a deamidation reaction on glutamine and asparagine residues rendering its biological hormone
functionality. Two Reverse Phase High Performance Liquid Chromatography (RP-HPLC) methods
were developed and validated for detection of insulin and degradant Asn A21 desamido insulin
(method A) and insulin and degradant Asn B3 desamido insulin (method B). Material biocompatibility
studies show that stainless steel and titanium are suitable for an implantable insulin delivery device
design over a 31-day period. The use of polycarbonate and polyurethane could be considered if the
insulin reservoir in the device was only to remain in the device for less than 11 days after which time
there is a loss in cresol which acts in a protective capacity for insulin stability. | en |
dc.language.iso | en | en |
dc.publisher | MDPI | en |
dc.subject | reverse phase HPLC, | en |
dc.subject | recombinant human insulin | en |
dc.subject | validation | en |
dc.subject | desamido insulin | en |
dc.subject | insulin stability | en |
dc.subject | Humulin R | en |
dc.title | Insulin Solution Stability and Biocompatibility with Materials Used for an Implantable Insulin Delivery Device Using Reverse Phase HPLC Methods | en |
dc.type | Article | en |
dc.identifier.doi | https://doi.org/10.3390/app9224794 | |
dc.peerreviewed | Yes | en |
dc.funder | Other external funder (please detail below) | en |
dc.cclicence | CC-BY-NC | en |
dc.date.acceptance | 2019-11-05 | |
dc.researchinstitute | Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI) | en |
dc.funder.other | The Edith Murphy Foundation | en |