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dc.contributor.authorOwen, Lucyen
dc.contributor.authorWhite, A.en
dc.contributor.authorLaird, Katieen
dc.date.accessioned2018-09-12T11:53:32Z
dc.date.available2018-09-12T11:53:32Z
dc.date.issued2018-10-02
dc.identifier.citationOwen, L., White, A. and Laird, K. (2018) Characterisation and screening of antimicrobial essential oil components against clinically important antibiotic-resistant bacteria using thin layer chromatography-direct bioautography hyphenated with GC-MS, LC-MS and NMR, Phytochemical Analysis, 30 (2), pp. 121-131en
dc.identifier.issn0958-0344
dc.identifier.urihttp://hdl.handle.net/2086/16577
dc.descriptionThe file attached to this record is the author's final peer reviewed version.en
dc.description.abstractIntroduction: The antimicrobial activity of many Essential Oils (EOs) is well established, indicating that EOs may be a source of compounds for antimicrobial drug development. Thin Layer Chromatography-Direct Bioautography (TLC-DB) can quickly identify antimicrobial components in complex mixtures and can be applied to the screening of EOs for lead compounds. Objectives: This study aimed to identify antimicrobial components of oregano, rosewood and cumin EOs against antibiotic-sensitive and -resistant bacteria using TLC-DB and a multi-faceted approach of GC-MS, LC-MS and NMR techniques to characterise bioactive compounds. The study also aimed to quantify the antimicrobial activity of bioactive compounds in order to evaluate their potential for the development of therapies against antibiotic-resistant bacteria. Materials and Methods: EOs were eluted on TLC plates and sprayed with a suspension of Staphylococcus aureus, Enterococcus faecium, Escherichia coli or Pseudomonas aeruginosa (antibiotic-sensitive and -resistant isolates). Zones of inhibition, visualised with iodonitrotetrazolium chloride, were subject to GC-MS, LC-MS and NMR to characterise the bioactive compounds. Results: Seven compounds were identified from the three EOs using GC-MS, while LC-MS and NMR failed to detect the presence of any further non-volatile or heat labile compounds. Carvacrol was most antimicrobial compound identified, with minimum inhibitory concentrations ranging 0.99-31.62 mM. Conclusion: The identified antimicrobial compounds present in oregano, rosewood and cumin EOs including carvacrol may be candidates for the development of novel antimicrobial therapies against antibiotic-resistant bacteria.en
dc.language.isoenen
dc.publisherWileyen
dc.titleCharacterisation and screening of antimicrobial essential oil components against clinically important antibiotic-resistant bacteria using thin layer chromatography-direct bioautography hyphenated with GC-MS, LC-MS and NMR.en
dc.typeArticleen
dc.identifier.doihttps://doi.org/10.1002/pca.2797
dc.researchgroupInfectious Disease Research Groupen
dc.peerreviewedYesen
dc.funderMicrobiology Societyen
dc.projectidN/Aen
dc.cclicenceCC-BY-NCen
dc.date.acceptance2018-09-06en
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en


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