Show simple item record

dc.contributor.authorAlfa, Hajaraen
dc.contributor.authorArroo, R. R. J.en
dc.contributor.authorWalsh, Susannah E.en
dc.contributor.authorRuparelia, K. C.en
dc.contributor.authorBhambra, Avninder S.en
dc.contributor.authorSlater, A.en
dc.date.accessioned2017-08-22T10:30:46Z
dc.date.available2017-08-22T10:30:46Z
dc.date.issued2017-07-01
dc.identifier.citationAlfa, H. et al. (2017) Pyrogallol is the main antibacterial compound in the aqueous extract of Boswellia dalzielii bark. Trends in Natural Product Research – PSE Young Scientists’ Meeting Lille 2017 Natural Products in Health, Agro-Food and Cosmetics – June 28th - July 1st, 2017en
dc.identifier.isbn9780956547262
dc.identifier.urihttp://hdl.handle.net/2086/14429
dc.description.abstractPlants belonging to the genus Boswellia (Burseraceae) have long been appreciated for their pharmacological properties such as anti-inflammatory, antioxidative and anticancer activities1. In the Northern part of Nigeria, the bark of B. dalzielii Hutch. is an important ingredient for the treatment of infections2. To identify water-soluble antibacterial compounds, powdered bark was macerated in water for 6 to 24h at a range of temperatures. The aqueous extracts were subsequently fractionated by column chromatography, and the fractions were initially screened against wild type and methicillin resistant strains of Staphylococcus aureus. Then, minimum inhibitory concentrations of purified fractions were determined using series of concentrations from 2 mg/mL to 8 μg/mL. Results showed that longer maceration resulted in stronger antibacterial activity. With the aid of NMR and accurate mass analysis, pyrogallol was identified as the main antibacterial agent, with MIC values ranging from 24-28 μg/mL for MRSA to 34-36 μg/mL for wild-type S. aureus. Gallic acid was found to play a lesser role (MIC >200 μg/mL). Pyrogallol was found not to be a plant secondary metabolite, but a metabolic product from microbial degradation of gallic acid from the bark. Only two bacterial species could be isolated from the plant material, which were identified as Raoultella planticola and Enterobacter cloacae. Of these two micro-organisms, R. planticola was shown to be responsible for the production of pyrogallol. This is an example of bacterial allelopathy, which results in an increased efficacy of the aqueous extract.en
dc.language.isoenen
dc.publisherPhytochemical Society of Europeen
dc.titlePyrogallol is the main antibacterial compound in the aqueous extract of Boswellia dalzielii barken
dc.typePresentationen
dc.researchgroupChemistry for Healthen
dc.peerreviewedYesen
dc.explorer.multimediaNoen
dc.funderN/Aen
dc.projectidN/Aen
dc.cclicenceN/Aen
dc.date.acceptance2017-07-28en
dc.researchinstituteInstitute for Allied Health Sciences Researchen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record