Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide
Glycosaminoglycans (GAGs) in the urine of Wistar rats, and on the surface of the epithelium and lamina propria of the bladder, were quantitatively assessed before and after acute intoxication with paraoxon or cyclophosphamide. Paraoxon was administered subcutaneously (s.c.) twice with an interval of 1 hour to a final dose of 275 mg/kg; cyclophosphamide was administered intraperitoneally (i.p.) with a single dose of 100 mg/kg or to a final dose of 240 mg/kg (three times per 80 mg/kg every 12 hours). GAGs on the surface of the epithelium and lamina propria of the urinary bladder of rats were quantitatively determined by Alcian blue dye staining. GAGs in the urine were determined spectrophotometrically with 1-9-dimethyl methylene blue. By 48-96 hours after intoxication with either paraoxon or cyclophosphamide, statistically significant increases in the concentration of GAGs were obtained both for the tissues of the bladder and the urine of rats. Cyclophosphamide, in contrast to paraoxon, caused the development of hemorrhagic cystitis in the animals. The synthesis of GAGs is considered to be compensatory response to the toxic xenobiotics.
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Citation : Sobolev, V. E., Jenkins, R.O. and Goncharov, N. (2017) Sulfated glycosaminoglycans in bladder tissue and urine of rats after acute exposure to paraoxon and cyclophosphamide. Experimental and Toxicologic Pathology, 69 (6), pp. 339-347
ISSN : 0940-2993
Research Group : Biomedical and Environmental Health
Research Institute : Institute for Allied Health Sciences Research
Peer Reviewed : Yes