Hydrogels based on copolymers of 2-hydroxyethylmethacrylate and 2-hydroxyethylacrylate as a delivery system for proteins: interactions with lysozyme
Hydrogels have attracted considerable attention due to numerous applications, in particular as contact lenses and carriers for sustained drug delivery. The aim of the present work is to characterize the interactions of copolymer hydrogels consisted of 2-hydroxyethylmethacrylate (HEMA) and 2-hydroxyethylacrylate (HEA) with a small protein (lysozyme) and to assess the potential applications of these hydrogels as a drug delivery system for sustained release of protein-based therapeutics. Physicochemical properties of protein-loaded hydrogels, as well as lysozyme in vitro loading and release and the conformation of the protein released from hydrogels were studied. The effect of copolymer composition on the protein deposition on hydrogels and protein aggregation in the presence of hydrogels was also assessed. The results show that introduction of HEA into the copolymeric hydrogels enhances their suitability as a delivery system for proteins. Copolymerisation of HEMA and HEA allows controlling the physicochemical properties of hydrogels and the protein release rate.
The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.
Citation : Hackl, E. V., Khutoryanskiy, V.V. and Ermolina, I. (2017) Hydrogels based on copolymers of 2-hydroxyethylmethacrylate and 2-hydroxyethylacrylate as a delivery system for proteins: interactions with lysozyme. Journal of Applied Polymer Science, 134 (18)
Research Group : Pharmaceutical Technologies
Research Institute : Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)
Peer Reviewed : Yes
- Leicester School of Pharmacy