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dc.contributor.authorAndjus, P. R.en
dc.contributor.authorBajic, A.en
dc.contributor.authorZhu, Lanen
dc.contributor.authorSchachner, M.en
dc.contributor.authorStrata, Piergiorgioen
dc.date.accessioned2015-04-29T13:23:02Z
dc.date.available2015-04-29T13:23:02Z
dc.date.issued2005-09-12
dc.identifier.citationAndjus, P.R. et al. (2005) Short-term facilitation and depression in the cerebellum: some observations on wild-type and mutant rodents deficient in the extracellular matrix molecule tenascin C. Annals of the New York Academy of Sciences, 1048, pp. 185-197.en
dc.identifier.issn0077-8923
dc.identifier.urihttp://hdl.handle.net/2086/10921
dc.description.abstractShort-term plasticity was studied on synapses to Purkinje cells (PC): paired-pulse facilitation in parallel fibers (PF) and paired-pulse depression in climbing fibers (CF). Both phenomena relate to synaptic strength. These forms of short-term plasticity were tested on cerebellar slices in rat by early postnatal synchronous stimulation of olivary neurons (i.e., CFs) with harmaline and by inhibition of a metabotropic glutamate receptor (mGluR) as well as in mice that were deficient in the extracellular matrix glycoprotein tenascin-C. Harmaline stimulation delayed the developmental competition between CF inputs and maintained multiple innervation. Paired-pulse depression of the CF-PC synapse after harmaline treatment was more expressed. However, paired-pulse facilitation in PF-PC synapses remained unchanged. Electrophysiological responses of postsynaptic mGluR1 in CF-PC synapses could be obtained only with AMPA receptors blocked and glutamate uptake impaired. The mGluR1-specific antagonist CPCCOEt suppressed the CF-mGluR EPSC in some PCs and potentiated it in other PCs. CF paired-pulse depression was not changed with CPCCOEt, thus excluding a presynaptic effect. The postsynaptic effect was underlined by CPCCOEt-induced rise in amplitude of EPSC and by a prolongation of its decay time. Tenascins are extracellular matrix glycoproteins that may restrict the regenerative capacity of the nervous tissue. Testing short-term presynaptic plasticity in tenascin-C-deficient mice showed that CF paired-pulse depression was less expressed while PF paired-pulse facilitation was augmented except in a group of cells where there was even depression. The results underline differences in forms of short-term plasticity with regard to susceptibility to diverse modulatory factors.en
dc.language.isoenen
dc.publisherAnnals of the New York Academy of Sciencesen
dc.subjectcerebellumen
dc.subjectPurkinje cellen
dc.subjectshort-term plasticityen
dc.subjecttenascinen
dc.subjectmetabotropic glutamate receptoren
dc.titleShort-term facilitation and depression in the cerebellum: some observations on wild-type and mutant rodents deficient in the extracellular matrix molecule tenascin Cen
dc.typeArticleen
dc.identifier.doihttp://dx.doi.org/10.1196/annals.1342.017
dc.peerreviewedYesen
dc.funderMIURen
dc.funderItalian Ministry of Healthen
dc.funderGrant 1647 of the Ministry of Science and Environmental Protection, Republic of Serbiaen
dc.projectidResearch supporten
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en


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