• Login
    View Item 
    •   DORA Home
    • Faculty of Health and Life Sciences
    • Leicester School of Pharmacy
    • View Item
    •   DORA Home
    • Faculty of Health and Life Sciences
    • Leicester School of Pharmacy
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The subcellular sites of sphingomyelin synthesis in BHK cells

    Thumbnail
    Date
    1997
    Author
    Obradors, M.;
    Sillence, Daniel J.;
    Howitt, S.;
    Allan, D.
    Metadata
    Show attachments and full item record
    Abstract
    The subcellular distributions of the enzymes which synthesise sphingomyelin (SM) and glucosylceramide (GluCer) from ceramide have been assessed in BHK cells. On a sucrose density gradient GluCer synthase (a marker of the cis/medial Golgi apparatus) and the trans-Golgi marker galactosyltransferase showed an similar monotonic distribution. In contrast, SM synthase showed two peaks of activity, a minor one which migrated with the Golgi markers and a major one which had a density close to that of plasma membrane markers (sphingomyelin, cholesterol, PtdSer, ganglioside GM3 and alkaline phosphodiesterase). When cell homogenates were treated with digitonin, the sedimentation characteristics of the Golgi markers was largely unaffected whereas the plasma membrane markers and the main peak of SM synthase activity were shifted to higher density. In contrast, when cells were treated with brefeldin A (BFA) the Golgi markers were shifted to higher density but not the plasma membrane markers or the main peak of SM synthase. These results suggest that the bulk of SM synthase activity in BHK cells is not associated with the Golgi cisternae but with a cell compartment which is relatively rich in cholesterol (e.g., plasma membrane, endosomes or trans-Golgi network.) Further experiments in which cells were treated with sphingomyelinase provided evidence that SM synthase activity was in an internal compartment and not at the plasma membrane.
    Description
    Citation : Obradors, M et al. (1997) The subcellular sites of sphingomyelin synthesis in BHK cells. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1359 (1), pp. 1-12
    URI
    http://hdl.handle.net/2086/10764
    DOI
    http://dx.doi.org/10.1016/S0167-4889(97)00088-8
    Research Institute : Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)
    Peer Reviewed : Yes
    Collections
    • Leicester School of Pharmacy [1196]

    Submission Guide | Reporting Guide | Reporting Tool | DMU Open Access Libguide | Take Down Policy | Connect with DORA
    DMU LIbrary
     

     

    Browse

    All of DORACommunities & CollectionsAuthorsTitlesSubjects/KeywordsResearch InstituteBy Publication DateBy Submission DateThis CollectionAuthorsTitlesSubjects/KeywordsResearch InstituteBy Publication DateBy Submission Date

    My Account

    Login

    Submission Guide | Reporting Guide | Reporting Tool | DMU Open Access Libguide | Take Down Policy | Connect with DORA
    DMU LIbrary