Browsing by Author "Savelieva, E. I."

Now showing 1 - 7 of 7
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    Determination of fluoroacetic acid in water and biological samples by GC-FID and GC-MS in combination with solid-phase microextraction.
    (Springer Verlag, 2006-11-01) Jenkins, R. O.; Khlebnikova, N. S.; Koryagina, N. L.; Savelieva, E. I.; Goncharov, Nikolay V.; Radilov, Andrey S.
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    Fluroacetate
    (Elsevier, 2009) Goncharov, Nikolay V.; Glashkina, L.; Savelieva, E. I.; Zinchenko, V.; Teplova, V. V.; Vinokurov, M.; Kuznetsov, S.; Mindukshev, I. V.; Avdonin, P.; Jenkins, R. O.; Radilov, Andrey S.
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    Fluroacetate.
    (Elsevier, 2015) Goncharov, Nikolay V.; Savelieva, E. I.; Zinchenko, V.; Kuznetsov, S.; Mindukshev, I. V.; Vinokurov, M.; Avdonin, P.; Voitenko, Natalia G.; Ukolov, A.; Orlova, O. I.; Jenkins, R. O.; Kuznetsov, A.
    Action of fluoroacetate (FA) becomes apparent after a latent period, even after exposure to lethal doses. The best known representative of FA is its sodium salt. There are also series of fluorinated compounds which metabolism is connected with formation of FA. To reveal intoxications, adequate analytical procedures should be developed, as well as unequivocal biochemical, physiological and clinical signs of intoxication should be described. Also, new approaches to development of an effective therapy for treating acute intoxications by FA are highly important, because after many decades of toxicological studies on FA effective antidotes are not available. These and other points are the subjects of discussion in this chapter. Some old and new data are critically revised, together with presentation of the authors’ experimental results, the main theme of which is development of new and effective therapy for treating acute intoxication by FA.
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    High-sensitivity determination of 2-chlorovinylarsonous acid in biomedical samples for retrospective detection of exposure to lewisite upon antidotal therapy.
    (IOS Press, 2011) Koryagina, N. L.; Ukolova, E. S.; Savelieva, E. I.; Voitenko, Natalia G.; Orlova, O. I.; Jenkins, R. O.; Goncharov, Nikolay V.
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    Microplate spectroscopic methods for determination of the organophosphate soman.
    (The Royal Society of Chemistry, 2010-12) Prokofieva, Daria S.; Voitenko, Natalia G.; Gustyleva, Lyudmila K.; Babakov, Vladimir N.; Savelieva, E. I.; Jenkins, R. O.; Goncharov, Nikolay V.
    Two microplate spectroscopic methods for determination of organophosphates, based on inhibition of acetylcholinesterase activity, have been elaborated and evaluated for determination of the chemical weapon agent soman. The principal difference between the methods is that one measures reaction substrate concentration (elaborated from Hestrin), while the other measures reaction product (elaborated from Ellman). The linear ranges of the two methods were found to be similar. Although the limit of quantification was lower for the Ellman method (110 pM), the sensitivity coefficient was in favor of the Hestrin method (1.55-fold higher). The effects of the main soman hydrolysis products were consistent for the two methods: both methylphosphonic acid and pinacolyl methylphosphonic acid did not inhibit acetylcholinesterase activity. The main components of decontaminating solutions showed differential effects: while monoethanolamine had no influence upon results obtained by either method, hydrogen peroxide interfered with the Ellman method at far lower concentrations than with the Hestrin method. In practical applications involving samples containing hydrogen peroxide, the method based on Hestrin should be regarded as much more specific for OP determination than the Ellman method.
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    Russian VX
    (Elsevier, 2015) Rembovskiy, V.; Radilov, Andrey S.; Savelieva, E. I.; Kuznetsov, S.; Mindukshev, I. V.; Prokofieva, Daria S.; Krivorotova, N.; Khlebnikova, N. S.; Jenkins, R. O.; Goncharov, Nikolay V.
    Presented in this chapter are data on the principal hydrolysis products of Russian VX (RVX) with excess and equimolar quantity of water, as well as a generalized list of compounds extracted from samples of construction materials of a former RVX production facility. Toxicokinetic experiments have been conducted to determine decrease of O-alkyl methylphosphonates in blood plasma of rats after i.m. administration of RVX. MS/MS peptide spectra obtained with commercially available human albumin incubated with RVX revealed covalent binding of RVX to Tyr 411 and Tyr150. A new covalent adduct of N,N-diethyl-2-aminoethanthiol (a RVX hydrolysis product) with blood plasma proteins has been detected by HPLC–MS/MS, and this can be another marker of exposure to RVX. New data on effects developed under chronic exposure of rats to RVX are presented: these are kinetic parameters of blood platelet aggregation and electrophysiology of Nervus tibialis. According to these data, morphofunctional changes at the level of the microcirculatory bed may prove to be a significant factor in the etiology of delayed effects.
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    Russian VX: monitoring and toxicity
    (Elsevier, 2009) Radilov, Andrey S.; Rembovskiy, V.; Rybalchenko, I.; Savelieva, E. I.; Podolskaya, E.; Babakov, Vladimir N.; Ermolaeva, E. E.; Dulov, S.; Kuznetsov, S.; Mindukshev, I. V.; Shpak, A.; Krasnov, I.; Khlebnikova, N. S.; Jenkins, R. O.; Goncharov, Nikolay V.