The development and evaluation of a multiplex real-time PCR assay for the detection of ESBL genes in urinary tract infections
dc.cclicence | CC-BY | en |
dc.contributor.author | Reid, R. | en |
dc.contributor.author | Samarasinghe, S. | en |
dc.date.acceptance | 2018-08-08 | en |
dc.date.accessioned | 2018-08-30T12:42:30Z | |
dc.date.available | 2018-08-30T12:42:30Z | |
dc.date.issued | 2018-08-09 | |
dc.description | open access journal | en |
dc.description.abstract | Background Overuse of beta-lactam antibiotics has lead to selection for extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae, a major cause of antibiotic resistant urinary tract infections (UTIs). Standard detection methods are time-consuming, with disputed accuracy. This study describes a novel real-time PCR method to detect CTX-M, SHV, OXA and TEM. Methods 179 Enterobacteriaceae isolates from UTIs were collected from the Leicester Royal Infirmary, UK. A multiplex Plexor®-based real-time PCR assay detected ESBLs using their specific amplicon melting temperature, during each cycle, removing the need for a melt-curve analysis. Validation was achieved by end-point PCR and disk diffusion. Results The method was able to produce rapid and accurate results, achieving a sensitivity and specificity of 94.9% and 72% respectively, and the assay can differentiate between the different ESBL genes, with ease. Conclusions With further investigation, a Plexor®-based assay could form the basis of a high-throughput kit that health services could use to detect ESBLs or other antibiotic resistance genes. | en |
dc.funder | N/A | en |
dc.identifier.citation | Reid, R. and Samarasinghe, S. (2018) The development and evaluation of a multiplex real-time PCR assay for the detection of ESBL genes in urinary tract infections. International Journal of Clinical Microbiology, 1 (1), pp. 16-24 | en |
dc.identifier.doi | https://doi.org/10.14302/issn.2690-4721.ijcm-18-2217 | |
dc.identifier.uri | https://oap-lifescience.org/ijcm/article/808 | |
dc.identifier.uri | http://hdl.handle.net/2086/16517 | |
dc.language.iso | en | en |
dc.peerreviewed | Yes | en |
dc.projectid | N/A | en |
dc.publisher | Open Access Pub | en |
dc.researchgroup | Infectious Disease Research Group | en |
dc.researchinstitute | Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI) | en |
dc.subject | Extended-spectrum beta-lactamases | en |
dc.subject | urinary tract infections | en |
dc.subject | Real-Time PCR | en |
dc.subject | rapid diagnostics | en |
dc.subject | antibiotic resistance | en |
dc.title | The development and evaluation of a multiplex real-time PCR assay for the detection of ESBL genes in urinary tract infections | en |
dc.type | Article | en |
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