Short-term facilitation and depression in the cerebellum: some observations on wild-type and mutant rodents deficient in the extracellular matrix molecule tenascin C

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dc.contributor.authorAndjus, P. R.en
dc.contributor.authorBajic, A.en
dc.contributor.authorZhu, Lanen
dc.contributor.authorSchachner, M.en
dc.contributor.authorStrata, Piergiorgioen
dc.date.accessioned2015-04-29T13:23:02Z
dc.date.available2015-04-29T13:23:02Z
dc.date.issued2005-09-12
dc.description.abstractShort-term plasticity was studied on synapses to Purkinje cells (PC): paired-pulse facilitation in parallel fibers (PF) and paired-pulse depression in climbing fibers (CF). Both phenomena relate to synaptic strength. These forms of short-term plasticity were tested on cerebellar slices in rat by early postnatal synchronous stimulation of olivary neurons (i.e., CFs) with harmaline and by inhibition of a metabotropic glutamate receptor (mGluR) as well as in mice that were deficient in the extracellular matrix glycoprotein tenascin-C. Harmaline stimulation delayed the developmental competition between CF inputs and maintained multiple innervation. Paired-pulse depression of the CF-PC synapse after harmaline treatment was more expressed. However, paired-pulse facilitation in PF-PC synapses remained unchanged. Electrophysiological responses of postsynaptic mGluR1 in CF-PC synapses could be obtained only with AMPA receptors blocked and glutamate uptake impaired. The mGluR1-specific antagonist CPCCOEt suppressed the CF-mGluR EPSC in some PCs and potentiated it in other PCs. CF paired-pulse depression was not changed with CPCCOEt, thus excluding a presynaptic effect. The postsynaptic effect was underlined by CPCCOEt-induced rise in amplitude of EPSC and by a prolongation of its decay time. Tenascins are extracellular matrix glycoproteins that may restrict the regenerative capacity of the nervous tissue. Testing short-term presynaptic plasticity in tenascin-C-deficient mice showed that CF paired-pulse depression was less expressed while PF paired-pulse facilitation was augmented except in a group of cells where there was even depression. The results underline differences in forms of short-term plasticity with regard to susceptibility to diverse modulatory factors.en
dc.funderMIURen
dc.funderItalian Ministry of Healthen
dc.funderGrant 1647 of the Ministry of Science and Environmental Protection, Republic of Serbiaen
dc.identifier.citationAndjus, P.R. et al. (2005) Short-term facilitation and depression in the cerebellum: some observations on wild-type and mutant rodents deficient in the extracellular matrix molecule tenascin C. Annals of the New York Academy of Sciences, 1048, pp. 185-197.en
dc.identifier.doihttps://doi.org/10.1196/annals.1342.017
dc.identifier.issn0077-8923
dc.identifier.urihttp://hdl.handle.net/2086/10921
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidResearch supporten
dc.publisherAnnals of the New York Academy of Sciencesen
dc.researchinstituteLeicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)en
dc.subjectcerebellumen
dc.subjectPurkinje cellen
dc.subjectshort-term plasticityen
dc.subjecttenascinen
dc.subjectmetabotropic glutamate receptoren
dc.titleShort-term facilitation and depression in the cerebellum: some observations on wild-type and mutant rodents deficient in the extracellular matrix molecule tenascin Cen
dc.typeArticleen

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