Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

Simple item page
dc.cclicenceCC-BYen
dc.contributor.authorCourt, E.K.
dc.contributor.authorChaudhuri, R.R.
dc.contributor.authorKapoore, R.V.
dc.contributor.authorVilla, R.
dc.contributor.authorPandhal, J.
dc.contributor.authorBiggs, C.A.
dc.contributor.authorStafford, G.P.
dc.date.acceptance2021-10-12
dc.date.accessioned2021-12-08T11:23:28Z
dc.date.available2021-12-08T11:23:28Z
dc.date.issued2021-12-06
dc.descriptionopen access article.en
dc.description.abstractSewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment. In this study we developed a pipeline for isolation of lipolytic strains directly from two FOG blockage sites in the UK, and isolated a range of highly lipolytic bacteria. We selected the five most lipolytic strains using Rhodamine B agar plates and pNP-Fatty acid substrates, with two Serratia spp., two Klebsiella spp. and an environmental Acinetobacter strain that all have the capacity to grow on FOG-based carbon sources. Their genome sequences identified the genetic capacity for fatty acid harvesting (lipases), catabolism and utilization (Fad genes). Furthermore, we performed a preliminary molecular characterization of the microbial community at these sites, showing a diverse community of environmental bacteria at each site, but which did include evidence of sequences related to our isolates. This study provides proof of concept to isolation strategies targeting Fatberg sites to yield candidate strains with bioremediation potential for FOG in the wastewater network. Our work sets the foundation for development of novel bioadditions tailored to the environment with non-pathogenic Acinetobacter identified as a candidate for this purpose.en
dc.funderEPSRC (Engineering and Physical Sciences Research Council)en
dc.funder.otherUniversity of Sheffield Impact and Innovation Knowledge Exchange (156922)en
dc.funder.otherUK Biotechnology and Biological Sciences Research Councilen
dc.identifier.citationCourt, E.K., Chaudhuri, R.R., Kapoore, R. V., Villa, R., Pandhal, J., Biggs, C.A. and Stafford G.P. (2021) Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site. Microbiology, 167(12).en
dc.identifier.doihttps://doi.org/10.1099/mic.0.001117
dc.identifier.issn1350-0872
dc.identifier.urihttps://hdl.handle.net/2086/21532
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidBB/L024209/1en
dc.projectidEP/N010124/1en
dc.projectidgrant number BB/L024209/1en
dc.publisherMicrobiology Societyen
dc.researchinstituteInstitute of Energy and Sustainable Development (IESD)en
dc.subjectFat oil and greaseen
dc.subjectFFAen
dc.subjectfree fatty acidsen
dc.subjectFOGen
dc.subjectSWWaen
dc.subjectsynthetic wastewateren
dc.titleLooking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg siteen
dc.typeArticleen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Court et al. Microbiology, 2021.pdf
Size:
2.22 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
4.2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

School of Engineering and Sustainable Development