Glycyrrhiza glabra extract and quercetin reverses cisplatin resistance in triple-negative MDA-MB-468 breast cancer cells via inhibition of cytochrome P450 1B1 enzyme

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dc.cclicenceCC-BY-NCen
dc.contributor.authorSharma, Rajnien
dc.contributor.authorGatchie, Lindaen
dc.contributor.authorWilliams, Ibidapo Stevenen
dc.contributor.authorShreyans, K. J.en
dc.contributor.authorVishwakarma, R. A.en
dc.contributor.authorChaudhuri, Bhabatoshen
dc.contributor.authorBharate, Sandip B.en
dc.date.acceptance2017-07-06en
dc.date.accessioned2018-02-08T14:43:25Z
dc.date.available2018-02-08T14:43:25Z
dc.date.issued2017-11-07
dc.description.abstractThe development of multi-drug resistance to existing anticancer drugs is one of the major challenges in cancer treatment. The over-expression of cytochrome P450 1B1 enzyme has been reported to cause resistance to cisplatin. With an objective to discover cisplatin-resistance reversal agents, herein, we report the evaluation of Glycyrrhiza glabra (licorice) extracts and its twelve chemical constituents for inhibition of CYP1B1 (and CYP1A1) enzyme in Sacchrosomes and live human cells. The hydroalcoholic extract showed potent inhibition of CYP1B1 in both Sacchrosomes as well as in live cells with IC50 values of 21 and 16 mg/ mL, respectively. Amongst the total of 12 constituents tested, quercetin and glabrol showed inhibition of CYP1B1 in live cell assay with IC50 values of 2.2 and 15 mM, respectively. Both these natural products were found to be selective inhibitors of CYP1B1, and does not inhibit CYP2 and CYP3 family of enzymes (IC50 > 20 mM). The hydroalcoholic extract of G. glabra and quercetin (4) showed complete reversal of cisplatin resistance in CYP1B1 overexpressing triple negative MDA-MB-468 breast cancer cells. The selective inhibition of CYP1B1 by quercetin and glabrol over CYP2 and CYP3 family of enzymes was studied by molecular modeling studies.en
dc.funderN/Aen
dc.identifier.citationSharma, R. et al. (2017) Glycyrrhiza glabra extract and quercetin reverses cisplatin resistance in triple-negative MDA-MB-468 breast cancer cells via inhibition of cytochrome P450 1B1 enzyme. Bioorganic and Medicinal Chemistry Letters, 27(24). pp.5400-5403.en
dc.identifier.doihttps://doi.org/10.1016/j.bmcl.2017.11.013
dc.identifier.urihttp://hdl.handle.net/2086/15191
dc.language.isoenen
dc.peerreviewedYesen
dc.projectidN/Aen
dc.publisherElsevieren
dc.subjectCisplatin-resistanceen
dc.subjectCYP1B1en
dc.subjectGlycyrrhiza glabraen
dc.subjectQuercetinen
dc.subjectGlabrolen
dc.subjectMDA-MB-468 cellsen
dc.titleGlycyrrhiza glabra extract and quercetin reverses cisplatin resistance in triple-negative MDA-MB-468 breast cancer cells via inhibition of cytochrome P450 1B1 enzymeen
dc.typeArticleen

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