Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells
The localization of phorbol ester-sensitive phospholipase D (PLD) in baby hamster kidney cells has been investigated by determining the subcellular distribution of the phosphatidylbutanol produced when the cells are incubated with phorbol 12-myristate 13-acetate and n-butanol. Results derived by isolation of plasma membrane vesicles from intact cells or by subcellular fractionation on a sucrose density gradient suggest the PLD is specific for phosphatidylcholine and its primary site of action is not the plasma membrane but the endoplasmic reticulum.
Citation : Decker, C. et al. (1996) Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells. Biochemical Journal, 320 (3), pp. 885-890
Research Institute : Leicester Institute for Pharmaceutical Innovation - From Molecules to Practice (LIPI)
Peer Reviewed : Yes
- Leicester School of Pharmacy